Formation, Fusion, and Regeneration of Protoplasts from Wild-Type and Auxotrophic Strains of the White Rot Basidiomycete Phanerochaete chrysosporium.
نویسندگان
چکیده
A preparation of two commercial enzymes was used to liberate protoplasts from 16-h-old mycelium of Phanerochaete chrysosporium. Regeneration frequencies of up to 5% were attained when the protoplasts were plated in a medium containing 10% sorbose and 3% agar. Fusion of protoplasts from different auxotrophic strains in polyethylene glycol-Ca produced heterokaryons. Separation of the heterokaryons into their constituent homokaryotic strains could be effected through protoplast release and formation of colonies on regeneration agar.
منابع مشابه
Characterization of Leucine Auxotrophs of the White Rot Basidiomycete Phanerochaete chrysosporium.
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 46 1 شماره
صفحات -
تاریخ انتشار 1983